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Materials and methods Animals Wistar albino male rats (aged 12 weeks, 350400 g b.w.) were bred in-house (the animal facility at the Department of Pharmacology, School of Medicine, Zagreb, Croatia

Upregulation of iron transport proteins such as transferrin (Tf), transferrin receptor (TfR), and divalent metal transporter 1 (DMT1) enhances cellular iron uptake, while loss or dysfunction of ferroportin 1 (FPN1) hampers iron efflux, leading to intracellular iron overload and oxidative damage